|
|
Susan Hamilton
Baylor College of MedicineDepartment: Molecular Physiology and BiophysicsAddress: One Baylor Plaza, Suite 410B Houston, TX 77030 Phone: 713-798-5704 Fax: 713-798-5441 Email: susanh@bcm.tmc.edu Web: www.bcm.edu/physio/hamilton/?pmid=6654 |
Education
B.S. Chemistry, Indiana University (1971)
Ph.D. Biophysics, University of Colorado Medical Center (1976)
Honors
Research Topic
Calcium Release Channel Structure and Function
Research Description
Depolarization of skeletal muscle fibers induces the release of Ca2+from intracellular stores. The process whereby the change in membrane potentialof the transverse tubule membranes triggers the opening of calcium channelsin the intracellular sarcoplasmic reticulum membranes is designated excitation-contractioncoupling. The research goal of my laboratory is to define the molecularevents involved in this process. Our approach is to characterize biochemically,pharmacologically, electrophysiologically and structurally the proteinsinvolved in this process. The two proteins which we study are the transversetubular dihydropyridine binding protein (which is the voltage sensor and/ora voltage dependent Ca2+ channel) and the sarcoplasmic reticulumryanodine binding protein (which is also a Ca2+ channel). Experimentallywe analyze the ligand binding properties of these proteins, we purify themand study their subunit structure and their interactions with other skeletalmuscle proteins, examine their ability to function as ion channels by reconstitutioninto planar lipid bilayers, and analyze their structure by electron cryomicroscopyand angular reconstitution.
Selected Publications
- Zhang, H., Zhang, J.-Z., Danila, C.I. and Hamilton, S.L. (2003). A noncontiguous, inter-subunit binding site for calmodulin on the skeletal muscle Ca2+ release channel. J. Biol. Chem. 278: 8348-8355.
- Serysheva, I.I., Ludtke,S.J., Baker, M.R., Chiu , W. and Hamilton, S.L. (2002). Structure of the voltage-gated L-type Ca2+ channel by electron cryomicroscropy. Proc. Natl. Acad. Sci USA. 99: 10370-10375.
- Baker, M.L., Serysheva, I.I., Sencer, S., Ludtke, S.J., Jiang, W., Hamilton, S.L. and Chiu, W. (2002). The skeletal muscle Ca2+ release channel has an oxidoreductase-like domain. Proc. Natl. Acad. Sci. USA 99: 12155-12160.
- Tang, W., Sencer, S., and Hamilton, S.L. (2002). Calmodulin Modulation of Proteins Involved in Excitation-Contraction Coupling. Frontiers in Bioscience 7: 1583-9.
- Sencer, S., Papineni, R., Halling, D.B., Zhang, J.Z., Pate, P., Krol, J. and Hamilton, S.L. (2001). Coupling on RYR1 and L-Type Calcium Channels Via Calmodulin Binding Domains. J. Biol. Chem. 276: 38237-38241.
- Serysheva, I.I., Schatz, M., van Heel, M., Chiu, W. and Hamilton, S.L. (1999). Structure of the Skeletal Muscle Calcium Release Channel Activated with Ca2+ and AMP-PCP. Biophys. J. 77: 1936-44.
- Moore, C.P., Rodney, G., Zhang, J.-Z., Santacruz-Toloza, L., Strasburg, G.M. and Hamilton, S.L. (1999) Apocalmodulin and Ca2+ Calmodulin Bind to the Same Region on the Skeletal Muscle Ca2+ Release Channel. Biochemistry 38: 8532-8537.
- Serysheva, I.I., Schatz, M., van Heel, M., Chiu, W. and Hamilton, S.L. (1998). Ca2+-Induced Conformational Changes in the 3D Structure of the Skeletal Muscle Ca2+ Release Channel. Electron Microscopy I:733-4.
Lab Members
Lab Photos
Last edited on: September 21, 2009